Higher physical activity levels are related to faecal microbiota
diversity and composition in young adults

Lourdes Ortiz-Alvarez; Huiwen Xu; Samuel Ruiz-Campos; Francisco M. Acosta; Jairo H. Migueles; Ramiro Vilchez-Vargas; Alexander Link; Julio Plaza-Díaz; Angel Gil; Idoia Labayen; Jonatan R. Ruiz; Borja Martinez-Tellez

doi:10.5114/biolsport.2025.139850

eISSN: 2083-1862
ISSN: 0860-021X

Biology of Sport

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Original paper

Higher physical activity levels are related to faecal microbiota diversity and composition in young adults

Lourdes Ortiz-Alvarez

^{1, 2}

,

Huiwen Xu

^{1, 2}

,

Samuel Ruiz-Campos

^{3, 4}

,

Francisco M. Acosta

^{1, 5, 6, 7}

,

Jairo H. Migueles

^{1, 8}

,

Ramiro Vilchez-Vargas

⁹

,

Alexander Link

⁹

,

Julio Plaza-Díaz

^{2, 10, 11}

,

Angel Gil

^{2, 11, 12, 13}

,

Idoia Labayen

¹⁴

,

Jonatan R. Ruiz

^{1, 13}

,

Borja Martinez-Tellez

^{1, 3, 4, 15}

PROFITH (PROmoting FITness and Health through Physical Activity) Research Group, Sport and Health University Research Institute (iMUDS), Department of Physical and Sports Education, Faculty of Sport Sciences, University of Granada, Granada, Spain
Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, Granada, Spain
Department of Nursing, Physiotherapy and Medicine and SPORT Research Group (CTS-1024), CERNEP Research Center, University of Almería, Almería, Spain
Biomedical Research Unit, Torrecárdenas University Hospital, Almería, 04009, Spain
Turku PET Centre, University of Turku, Turku, Finland
Turku PET Centre, Turku University Hospital, Turku, Finland
InFLAMES Research Flagship Center, University of Turku, Turku, Finland
Department of Biosciences and Nutrition, Karolinska Institute, Karolinska, Sweden
Department of Gastroenterology, Hepatology and Infectious Diseases, Otto-von-Guericke-University Magdeburg, Magdeburg, Germany
Children’s Hospital of Eastern Ontario Research Institute, Ottawa, ON K1H 8L1, Canada
Institute of Nutrition and Food Technology “José Mataix”, Biomedical Research Center, Parque Tecnológico Ciencias de la Salud, University of Granada, Armilla, Granada, Spain
CIBEROBN, Biomedical Research Networking Center for Physiopathology of Obesity and Nutrition, Carlos III Health Institute, Madrid, Spain
Instituto de Investigación Biosanitaria, ibs.Granada, Granada, Spain
Institute for Sustainability & Food Chain Innovation (ISFOOD), Department of Health Sciences, Public University of Navarra, Campus de Arrosadía, Pamplona, Spain
Department of Medicine, Division of Endocrinology, and Einthoven Laboratory for Experimental Vascular Medicine, Leiden University Medical Center, Leiden, The Netherlands

Biol Sport. 2025;42(1):123–135

DOI: https://doi.org/10.5114/biolsport.2025.139850

Online publish date: 2024/06/04

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1. Myers J, Kokkinos P, Nyelin E. Physical activity, cardiorespiratory fitness, and the metabolic syndrome. Nutrients. 2019; 11(7):1652.

2. Rosenbaum M, Knight R, Leibel RL. The gut microbiota in human energy homeostasis and obesity. Trends Endocrinol Metab. 2015; 26(9):493–501.

3. Hooper L V., Gordon JI. Commensal host-bacterial relationships in the gut. Science. 2001; 292(5519):1115–1118.

4. Monda V, Villano I, Messina A, Valenzano A, Esposito T, Moscatelli F, Viggiano A, Cibelli G, Chieffi S, Monda M, Messina G. Exercise modifies the gut microbiota with positive health effects. Oxid Med Cell Longev. 2017; 2017:1–8.

5. Iebba V, Totino V, Gagliardi A, Santangelo F, Cacciotti F, Trancassini M, Mancini C, Cicerone C, Corazziari E, Pantanella F, Schippa S. Eubiosis and dysbiosis: The two sides of the microbiota. New Microbiol. 2016; 39(1):1–12.

6. David LA, Maurice CF, Carmody RN, Gootenberg DB, Button JE, Wolfe BE, Ling A V., Devlin AS, Varma Y, Fischbach MA, Biddinger SB, Dutton RJ, Turnbaugh PJ. Diet rapidly and reproducibly alters the human gut microbiome. Nature. 2014; 505(7484):559–563.

7. Moscatelli F, De Maria A, Marinaccio LA, Monda V, Messina A, Monacis D, Toto G, Limone P, Monda M, Messina G, Monda A, Polito R. Assessment of lifestyle, eating habits and the effect of nutritional education among undergraduate students in southern Italy. Nutrients. 2023; 15(13):1–13.

8. Castellanos N, Diez GG, Antúnez-Almagro C, Bailén M, Bressa C, González Soltero R, Pérez M, Larrosa M. A critical mutualism – Competition interplay underlies the loss of microbial diversity in sedentary lifestyle. Front Microbiol. 2020; 10:3142.

9. Maier L, Pruteanu M, Kuhn M, Zeller G, Telzerow A, Anderson EE, Brochado AR, Fernandez KC, Dose H, Mori H, Patil KR, Bork P, Typas A. Extensive impact of non-antibiotic drugs on human gut bacteria. Nature. 2018; 555(7698):623–628.

10. Kelly TN, Bazzano LA, Ajami NJ, He H, Zhao J, Petrosino JF, Correa A, He J. Gut microbiome associates with lifetime cardiovascular disease risk profile among bogalusa heart study participants. Circ Res. 2016; 119(8):956–964.

11. Ortiz-Alvarez L, Xu H, Martinez-Tellez B. Influence of exercise on the human gut microbiota of healthy adults: A systematic review. Clin Transl Gastroenterol. 2020; 11(2):1–9.

12. Mitchell CM, Davy BM, Hulver MW, Neilson AP, Bennett BJ, Davy KP. Does exercise alter gut microbial composition? A systematic review. Med Sci Sports Exerc. 2019; 51(1):160–167.

13. Clarke SF, Murphy EF, O’Sullivan O, Lucey AJ, Humphreys M, Hogan A, Hayes P, O’Reilly M, Jeffery IB, Wood-Martin R, Kerins DM, Quigley E, Ross RP, O’Toole PW, Molloy MG, Falvey E, Shanahan F, Cotter PD. Exercise and associated dietary extremes impact on gut microbial diversity. Gut. 2014; 63(12):1913–1920.

14. Barton W, Penney NC, Cronin O, Garcia-Perez I, Molloy MG, Holmes E, Shanahan F, Cotter PD, O’Sullivan O. The microbiome of professional athletes differs from that of more sedentary subjects in composition and particularly at the functional metabolic level. Gut. 2018; 67(4):625–633.

15. Petri C, Mascherini G, Izzicupo P, Rosati D, Cerboneschi M, Smeazzetto S, Arrones LS. Gut microbiota and physical activity level: characterization from sedentary to soccer players. Biol Sport. 2024; 41(3):169–176.

16. Bressa C, Bailén-Andrino M, Pérez-Santiago J, González-Soltero R, Pérez M, Montalvo-Lominchar MG, Maté-Muñoz JL, Domínguez R, Moreno D, Larrosa M. Differences in gut microbiota profile between women with active lifestyle and sedentary women. PLoS One. 2017; 12(2):1–20.

17. Khan TJ, Ahmed YM, Zamzami MA, Siddiqui AM, Khan I, Baothman OAS, Mehanna MG, Kuerban A, Kaleemuddin M, Yasir M. Atorvastatin treatment modulates the gut microbiota of the hypercholesterolemic patients. OMICS. 2018; 22(2):154–163.

18. Ruiz-Limón P, Muralidharan J, Gomez-Perez AM, Murri M, Vioque J, Corella D, Fitó M, Vidal J, Salas-Salvadó J, Torres-Collado L, Coltell O, Atzeni A, Castañer O, Bulló M, Bernal-López MR, Moreno-Indias I, Tinahones FJ. Physical activity shifts gut microbiota structure in aged subjects with overweight/obesity and metabolic syndrome. Biol Sport. 2023; 41(3):47–60.

19. Hughes R, Burd N, Khan N, Pindus D, Holscher H. Associations between physical activity and gut microbiota composition in adults with overweight and obesity. Curr Dev Nutr. 2022; 6(Suppl 1):1011.

20. Shephard RJ. Limits to the measurement of habitual physical activity by questionnaires. Br J Sports Med. 2003; 37(3):197–206.

21. Sanchez-Delgado G, Martinez-Tellez B, Olza J, Aguilera CM, Labayen I, Ortega FB, Chillon P, Fernandez-Reguera C, Alcantara JMA, Martinez-Avila WD, Muñoz-Hernandez V, Acosta FM, Prados-Ruiz J, Amaro-Gahete FJ, Hidalgo-Garcia L, Rodriguez L, Ruiz YAK, Ramirez-Navarro A, Muros-de Fuentes MA, García-Rivero Y, Sanchez-Sanchez R, de Dios Beas Jimenez J, de Teresa C, Navarrete S, Lozano R, Brea-Gomez E, Rubio-Lopez J, Ruiz MR, Cano-Nieto A, Llamas-Elvira JM, Jimenez Rios JA, Gil A, Ruiz JR. Activating brown adipose tissue through exercise (ACTIBATE) in young adults: Rationale, design and methodology. Contemp Clin Trials. 2015; 45(Pt B):416–425.

22. Migueles JH, Cadenas-Sanchez C, Ekelund U, Delisle Nyström C, Mora-Gonzalez J, Löf M, Labayen I, Ruiz JR, Ortega FB. Accelerometer data collection and processing criteria to

23. assess physical activity and other outcomes: A systematic review and practical considerations. Sports Med. 2017; 47(9):1821–1845.

24. Migueles JH, Rowlands A V., Huber F, Sabia S, Van Hees VT. GGIR: A research community–driven open source R package for generating physical activity and sleep outcomes from multi-day raw accelerometer data. J Meas Phys Behav. 2019; 2(3):188–196.

25. Van Hees VT, Fang Z, Langford J, Assah F, Mohammad A, Da Silva ICM, Trenell MI, White T, Wareham NJ, Brage S. Autocalibration of accelerometer data for free-living physical activity assessment using local gravity and temperature: An evaluation on four continents. J Appl Physiol. 2014; 117(7):738–744.

26. Acosta FM, Martinez-Tellez B, Sanchez-Delgado G, Migueles JH, Contreras-Gomez MA, Martinez-Avila WD, Merchan-Ramirez E, Alcantara JMA, Amaro-Gahete FJ, Llamas-Elvira JM, Ruiz JR. Association of objectively measured physical activity with brown adipose tissue volume and activity in young adults. J Clin Endocrinol Metab. 2019; 104(2):223–233.

27. Van Hees VT, Sabia S, Anderson KN, Denton SJ, Oliver J, Catt M, Abell JG, Kivimäki M, Trenell MI, Singh-Manoux A. A novel, open access method to assess sleep duration using a wrist-worn accelerometer. PLoS One. 2015; 10(11):1–13.

28. Hildebrand M, Hansen BH, van Hees VT, Ekelund U. Evaluation of raw acceleration sedentary thresholds in children and adults. Scand J Med Sci Sports. 2017; 27(12):1814–1823.

29. Callahan BJ, McMurdie PJ, Rosen MJ, Han AW, Johnson AJA, Holmes SP. DADA2: High-resolution sample inference from Illumina amplicon data. Nat Methods. 2016; 13(7):581–583.

30. McMurdie PJ, Holmes S. Phyloseq: An R package for reproducible interactive analysis and graphics of microbiome census data. PLoS One. 2013; 8(4):1–11.

31. Wang Q, Garrity GM, Tiedje JM, Cole JR. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol. 2007; 73(16):5261–5267.

32. Cole JR, Wang Q, Fish JA, Chai B, McGarrell DM, Sun Y, Brown CT, Porras-Alfaro A, Kuske CR, Tiedje JM. Ribosomal database project: Data and tools for high throughput rRNA analysis. Nucleic Acids Res. 2014; 42(Database issue):D633–642.

33. Schulz C, Schütte K, Koch N, Vilchez-Vargas R, Wos-Oxley ML, Oxley APA, Vital M, Malfertheiner P, Pieper DH. The active bacterial assemblages of the upper GI tract in individuals with and without helicobacter infection. Gut. 2018; 67(2):216–225.

34. Finotello F, Mastrorilli E, Di Camillo B. Measuring the diversity of the human microbiota with targeted next-generation sequencing. Brief Bioinform. 2018; 19(4):679–692.

35. Lozupone CA, Knight R. Species divergence and the measurement of microbial diversity. FEMS Microbiol Rev. 2008; 32(4):557–578.

36. Leo Lahti, Sudarshan Shetty. Microbiome R package. 2019.

37. Gotelli NJ, Colwell RK. Estimating species richness. In: Biological diversity: Frontiers in measurement and assessment. Oxford, United Kingdom: Oxford University Press; 2011. p. 39–54.

38. Magurran A. E. Measuring biological diversity. Oxford, United Kingdom: Blackwell’s; 2004. p. 184–189.

39. Simpson E. H. Measurement of diversity. Nature. 1949; 163:688.

40. Camargo JA. New diversity index for assessing structural alterations in aquatic communities. Bull Environ Contam Toxicol. 1992; 48(3):428–434.

41. Ruiz López MD, Artacho Martín-Lagos R. Guía para estudios dietéticos: Álbum fotográfico de alimentos. 1st ed. Granada, Spain: Editorial Universidad de Granada; 2010. p. 124–128.

42. Sanchez-Delgado G, Martinez-Tellez B, Olza J, Aguilera CM, Labayen I, Ortega FB, Chillon P, Fernandez-Reguera C, Alcantara JMA, Martinez-Avila WD, Muñoz-Hernandez V, Acosta FM, Prados-Ruiz J, Amaro-Gahete FJ, Hidalgo-Garcia L, Rodriguez L, Ruiz YAK, Ramirez-Navarro A, Muros-de Fuentes MA, García-Rivero Y, Sanchez-Sanchez R, de Dios Beas Jimenez J, de Teresa C, Navarrete S, Lozano R, Brea-Gomez E, Rubio-Lopez J, Ruiz MR, Cano-Nieto A, Llamas-Elvira JM, Jimenez Rios JA, Gil A, Ruiz JR. Activating brown adipose tissue through exercise (ACTIBATE) in young adults: Rationale, design and methodology. Contemp Clin Trials. 2015; 45(Pt B):416–425.

43. William Revelle. Psych: Procedures for personality and psychological research. 2017.

44. Wei T, Simko V, Levy M, Xie Y, Jin Y, Zemla J, Freidank M, Cai J, Protivinsky T. R package corrplot: Visualization of a correlation matrix. 2022.

45. Kim YS, Unno T, Kim BY, Park MS. Sex differences in gut microbiota. World J Mens Health. 2020; 38(1):48–60.

46. Palmas V, Pisanu S, Madau V, Casula E, Deledda A, Cusano R, Uva P, Vascellari S, Loviselli A, Manzin A, Velluzzi F. Gut microbiota markers associated with obesity and overweight in Italian adults. Sci Rep. 2021; 11(1):5532.

47. Zmora N, Suez J, Elinav E. You are what you eat: diet, health and the gut microbiota. Nat Rev Gastroenterol Hepatol. 2019; 16(1):35–56.

48. Hammer Ø, Harper D. Paleontological data analysis. Malden, United States: Blackwell Publishing; 2006. p. 351.

49. Benjamini Y, Krieger AM, Yekutieli D. Adaptive linear step-up false discovery rate controlling procedures. Biometrika. 2006; 93(3):491–507.

50. Kulecka M, Fraczek B, Mikula M, Zeber-Lubecka N, Karczmarski J, Paziewska A, Ambrozkiewicz F, Jagusztyn-Krynicka K, Cieszczyk P, Ostrowski J. The composition and richness of the gut microbiota differentiate the top Polish endurance athletes from sedentary controls. Gut Microbes. 2020; 11(5):1374–1384.

51. Imdad S, So B, Jang J, Park J, Lee SJ, Kim JH, Kang C. Temporal variations in the gut microbial diversity in response to high-fat diet and exercise. Sci Rep. 2024; 14(1):3282.

52. Estaki M, Pither J, Baumeister P, Little JP, Gill SK, Ghosh S, Ahmadi-Vand Z, Marsden KR, Gibson DL. Cardiorespiratory fitness as a predictor of intestinal microbial diversity and distinct metagenomic functions. Microbiome. 2016; 4(1):1–13.

53. Laureto LMO, Cianciaruso MV, Samia DSM. Functional diversity: an overview of its history and applicability. Natureza & Conservação. 2015; 13(2):112–116.

54. Lozupone CA, Stombaugh JI, Gordon JI, Jansson JK, Knight R. Diversity, stability and resilience of the human gut microbiota. Nature. 2012; 489(7415):220–230.

55. Marttinen M, Ala-Jaakkola R, Laitila A, Lehtinen MJ. Gut microbiota, probiotics and physical performance in athletes and physically active individuals. Nutrients. 2020; 12(10):1–32.

56. Etxeberria U, Hijona E, Aguirre L, Milagro FI, Bujanda L, Rimando AM, Martínez JA, Portillo MP. Pterostilbene-induced changes in gut microbiota composition in relation to obesity. Mol Nutr Food Res. 2017; 61(1):1–37.

57. Bai J, Hu Y, Bruner DW. Composition of gut microbiota and its association with body mass index and lifestyle factors in a cohort of 7–18 years old children from the American Gut Project. Pediatr Obes. 2019; 14(4):1–10.

58. Morgan XC, Tickle TL, Sokol H, Gevers D, Devaney KL, Ward D V., Reyes JA, Shah SA, LeLeiko N, Snapper SB, Bousvaros A, Korzenik J, Sands BE, Xavier RJ, Huttenhower C. Dysfunction of the intestinal microbiome in inflammatory bowel disease and treatment. Genome Biol. 2012; 13(9):1–18.

59. Shin NR, Whon TW, Bae JW. Proteobacteria: Microbial signature of dysbiosis in gut microbiota. Trends Biotechnol. 2015; 33(9):496–503.

60. Yang Y, Shi Y, Wiklund P, Tan X, Wu N, Zhang X, Tikkanen O, Zhang C, Munukka E, Cheng S. The association between cardiorespiratory fitness and gut microbiota composition in premenopausal women. Nutrients. 2017; 9(8):792.

61. Baldanzi G, Sayols-Baixeras S, Ekblom-Bak E, Ekblom Ö, Dekkers KF, Hammar U, Nguyen D, Ahmad S, Ericson U, Arvidsson D, Börjesson M, Johanson PJ, Smith JG, Bergström G, Lind L, Engström G, Ärnlöv J, Kennedy B, Orho-Melander M, Fall T. Accelerometer-based physical activity is associated with the gut microbiota in 8416 individuals in SCAPIS. EBioMedicine. 2024; 100:104989.

62. Quiroga R, Nistal E, Estébanez B, Porras D, Juárez-Fernández M, Martínez-Flórez S, García-Mediavilla MV, de Paz JA, González-Gallego J, Sánchez-Campos S, Cuevas MJ. Exercise training modulates the gut microbiota profile and impairs inflammatory signaling pathways in obese children. Exp Mol Med. 2020; 52(7):1048–1061.

63. Munukka E, Ahtiainen JP, Puigbó P, Jalkanen S, Pahkala K, Keskitalo A, Kujala UM, Pietilä S, Hollmén M, Elo L, Huovinen P, D’Auria G, Pekkala S. Six-week endurance exercise alters gut metagenome that is not reflected in systemic metabolism in over-weight women. Front Microbiol. 2018; 9:1–16.

64. Liang R, Zhang S, Peng X, Yang W, Xu Y, Wu P, Chen J, Cai Y, Zhou J. Characteristics of the gut microbiota in professional martial arts athletes: A comparison between different competition levels. PLoS One. 2019; 14(12):1–13.

65. de Oliveira Neves VG, de Oliveira DT, Oliveira DC, Oliveira Perucci L, dos Santos TAP, da Costa Fernandes I, de Sousa GG, Barboza NR, Guerra-Sá R. High-sugar diet intake, physical activity, and gut microbiota crosstalk: Implications for obesity in rats. Food Sci Nutr. 2020; 8(10):5683–5695.

66. Liu TW, Park YM, Holscher HD, Padilla J, Scroggins RJ, Welly R, Britton SL, Koch LG, Vieira-Potter VJ, Swanson KS. Physical activity differentially affects the cecal microbiota of ovariectomized female rats selectively bred for high and low aerobic capacity. PLoS One. 2015; 10(8):1–17.

67. Fernández J, Fernández-Sanjurjo M, Iglesias-Gutiérrez E, Martínez-Camblor P, Villar CJ, Tomás-Zapico C, Fernández-García B, Lombó F. Resistance and endurance exercise training induce differential changes in gut microbiota composition in murine models. Front Physiol. 2021; 12.

68. Parker BJ, Wearsch PA, Veloo ACM, Rodriguez-Palacios A. The genus alistipes: Gut bacteria with emerging implications to inflammation, cancer, and mental health. Front Immunol. 2020; 11:1–15.

69. Wyatt M, Greathouse KL. Targeting dietary and microbial tryptophan-indole metabolism as therapeutic approaches to colon cancer. Nutrients. 2021; 13(4):1–23.

70. Migueles JH, Cadenas-Sanchez C, Tudor-Locke C, Löf M, Esteban-Cornejo I, Molina-Garcia P, Mora-Gonzalez J, Rodriguez-Ayllon M, Garcia-Marmol E, Ekelund U, Ortega FB. Comparability of published cut-points for the assessment of physical activity: Implications for data harmonization. Scand J Med Sci Sports. 2019; 29(4):566–574.

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